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A2942 Sigma

Amphotericin B solution

250 μg/mL in deionized water, sterile-filtered, BioReagent, suitable for cell culture



Related Categories Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antifungal,
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   250 μg/mL in deionized water
application(s)   cell culture | mammalian: suitable
color   yellow
suitability   suitable for cell culture
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
shipped in   dry ice
storage temp.   −20°C



This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.


Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.


Amphotericin B remains active for 3 days in culture at 37°C.



Preparation Note

It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Biochem/physiol Actions

Amphotericin B solution has been used-
• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

General description

Amphotericin B is a polyene antifungal antibiotic that is clinically used to treat systemic fungal infections.

Chemical structure: polyene

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Light sensitive.

Protocols & Applications

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Safety Information

NONH for all modes of transport

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How should Product A2942, Amphotericin B solution, be stored?
This product should be stored at -20 °C.  It is stable in culture at 37 °C up to 3 days.
At what concentration should Product A2942, Amphotericin B solution, be used?
We suggest a working concentration of 10 ml/L (2.5 mg/L).
What is the shelf life of Product A2942, Amphotericin B solution?
It has an expiration date of 18 months. This can be found on the lot-specific certificate of analysis.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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