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A2980 Sigma-Aldrich

Anti-AcV5 antibody, Mouse monoclonal

clone ACV5, purified from hybridoma cell culture

Synonym: Anti-Baculovirus envelope gp64 protein

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
biological source   mouse
antibody form   purified immunoglobulin
antibody product type   primary antibodies
clone   ACV5, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt ~58 kDa
concentration   ~1 mg/mL
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 0.5-1 μg/mL using extracts of Sf9 cells infected with baculovirus
isotype   IgG2b
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

AcNPV extracellular nonocculeded virus (NOV).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

Proteins tagged with this AcV5 peptide are functional in both dicot and monocot plants and show no cross reactivity across many plant species. Epitope tagged fusion proteins are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. Anti-AcV5 antibody, Mouse monoclonal, clone ACV5 may be used in immunoblotting, immunoprecipitation, dot blot, and immunocytochemistry.

General description

The antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein (efp). Proteins tagged with the AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.

gp64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. It is involved in involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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