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A2980 Sigma-Aldrich

Anti-AcV5 antibody, Mouse monoclonal

clone ACV5, purified from hybridoma cell culture

Synonym: Anti-Baculovirus envelope gp64 protein

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Plant Biology, Primary Antibodies More...
conjugate   unconjugated
clone   ACV5, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 0.5-1 μg/mL using extracts of Sf9 cells infected with baculovirus
mol wt   antigen ~58 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   purified immunoglobulin
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL

Description

General description

Anti-AcV5 antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the hybridoma ACV5 produced by the fusion of mouse myeloma cells and splenocytes from C57Bl/6 X Balb/c mice immunized with AcNPV extracellular nonoccluded virus (NOV). GP64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

gp64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. It is involved in involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types.

Immunogen

AcNPV extracellular nonocculeded virus (NOV).

Application

Anti-AcV5 antibody, Mouse monoclonal has been used in:
• immunoblotting
• immunoprecipitation
• dot blot
• immunocytochemistry

Proteins tagged with this AcV5 peptide are functional in both dicot and monocot plants and show no cross reactivity across many plant species. Epitope tagged fusion proteins are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. Anti-AcV5 antibody, Mouse monoclonal, clone ACV5 may be used in immunoblotting, immunoprecipitation, dot blot, and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

GP64 (AcV5) is involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types. AcV5 is a monoclonal antibody that recognizes a nine amino acid residue tag (SWKDASGWS) of baculovirus Autographa californica GP64 envelope fusion protein (efp).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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