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  • A3085 - 5-(N-Ethyl-N-isopropyl)amiloride

A3085 Sigma


Synonym: EIPA



Related Categories A to C, Adenosinetriphosphatase (Na/K), Amiloride and Derivatives, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules,
mp   202-205 °C(lit.)
storage temp.   2-8°C
Gene Information   human ... SCNN1A(6337), SCNN1B(6338), SCNN1D(6339), SCNN1G(6340), SLC9A1(6548), SLC9A5(6553)
mouse ... Scnn1a(20276), Scnn1b(20277), Scnn1d(140501), Scnn1g(20278)
rat ... Scnn1a(25122), Scnn1b(24767), Scnn1g(24768), Slc9a1(24782), Slc9a2(24783), Slc9a3(24784), Slc9a4(24785)


Biochem/physiol Actions

Selective blocker of Na+/H+ antiport


25, 100 mg in poly bottle

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

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Biomedical Applications

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Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport

Frequently Asked Questions

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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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How can I store a solution of  5-(N-Ethyl-N-isopropyl)amiloride, Product A3085?
We do not have solution stability information for  5-(N-Ethyl-N-isopropyl)amiloride.  The product is sensitive to light, particularly in solution. In general, drug solutions are best prepared immediately before use.  We recommend storing at -20°C in single use aliquots.
What can I use to make a solution of 5-(N-Ethyl-N-isopropyl)amiloride, Product A3085?
We test solubility of 5-(N-Ethyl-N-isopropyl)amiloride in methanol (10 mg/mL), yielding a clear yellow solution.  It is also soluble in DMSO, and in dilute aqueous acid.  It is insoluble in water.
What concentration of Product A3085, 5-(N-Ethyl-N-isopropyl)amiloride, should I use in my experiment?
We have the following references for the use of  5-(N-Ethyl-N-isopropyl)amiloride.  A review article mentions the use of EIPA at 1 micromolar to probe Na+ channels in rat alveolar epithelial cells; see: Matalon, S., et al., Biophysical and molecular properties of amiloride-inhibitable Na+ channels in alveolar epithelial cells. Am. J. Physiol., 271, L1-L22 (1996).  A study of the effects of EIPA on infarction in isolated rabbit hearts and cardiomyocytes has been reported; see: Sato, H., et al., The mechanism of protection from 5 (N-ethyl-N-isopropyl)amiloride differs from that of ischemic preconditioning in rabbit heart. Basic Res. Cardiol., 92, 339-350 (1997).
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Protocols & Articles


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