EMAIL THIS PAGE TO A FRIEND
A3157 Sigma-Aldrich

Alcian Blue 8GX

certified by the Biological Stain Commission

Synonym: Alcian Blue, Ingrain Blue 1

  • CAS Number 33864-99-2

  • Empirical Formula (Hill Notation) C56H68Cl4CuN16S4

  • Molecular Weight 1298.86

  •  Colour Index Number 74240

  •  EC Number 251-705-7

  •  MDL number MFCD00010720

  •  PubChem Substance ID 24890721

  •  NACRES NA.47

Purchase

Properties

Related Categories A, Buffers, Reagents, & Stains, Cell Biology, Certified Stains & Dyes, Core Bioreagents,
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, ≥50%
application(s)   hematology: suitable
  histology: suitable
solubility   water: 1 mg/mL, blue
εmax   195-400 at 617-630 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
InChI   1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
InChI key   CKLBXIYTBHXJEH-UHFFFAOYSA-J

Description

Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.

Packaging

10, 25 g in glass bottle

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Suitability

Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?