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A3221 Sigma-Aldrich

Iodoacetamide

Single use vial of 56 mg

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Properties

Related Categories Cell Lysis and Protein Extraction Reagents, Chemical Modification Reagents, Molecular Biology, Native Protein Sample Preparation, Protein Modification,
InChI Key   PGLTVOMIXTUURA-UHFFFAOYSA-N
assay   ≥99% (HPLC)
packaging   vial of 56 mg (Single use)
mp   92-95 °C (lit.)
solubility   H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
storage temp.   2-8°C

Description

Application

Iodoacetamide may be used as an alkylating reagent for cysteine residues in peptide sequencing and peptide/protein analytical analysis. It may be used in 2-D protein electrophoresis to reduce streaking and improve resolution by eliminating artifacts that could result from disulfide formation during electrophoresis. By virtue of its reaction with cysteine, iodoacetamide is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease. Iodoacetamide may be used in disulfide stress research.

Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.

Packaging

10 vials in glass insert

Packaged in sealed ampules

Legal Information

Technology developed in partnership with Proteome Systems

Proteome Systems is a trademark of Proteome Systems Ltd

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
3
RTECS 
AC4200000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Development of a Simple and Rapid Digestion Protocol for Proteomics Sample Preparation

Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation. Sample preparation workflows are often cumbersome and generally involve c...
Zhiyun Cao, Amber Henry, Judy Boland, Nicolas Caffarelli, Jeffrey Turner, Kevin Ray
Keywords: Alkylations, Cell disruption, Detergents, Digestions, Mass spectrometry, Nucleic acid denaturation, Proteomics, Reductions, Sample preparations

HPLC Analysis of Carboxyamidomethylated Lysozyme Tryptic Digest on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamidomethylated Lysozyme Tryptic Digest on BIOshell™ A160 Peptide C18
Keywords: Chromatography, Digestions, High performance liquid chromatography, Mass spectrometry

Peer-Reviewed Papers
15

References

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