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A3411 Sigma-Aldrich

Aminopterin

powder, BioReagent, suitable for cell culture

Synonym: (S)-2-{4-[(2,4-Diaminopteridin-6-yl)methylamino]benzamido}pentanedioic acid, 4-Amino-PGA, 4-Aminofolic acid, 4-Aminopteroyl-L-glutamic acid

  • CAS Number 54-62-6

  • Empirical Formula (Hill Notation) C19H20N8O5

  • Molecular Weight 440.41

  •  Beilstein/REAXYS Number 69045

  •  EC Number 200-209-9

  •  PubChem Substance ID 24890768

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Properties

Related Categories Cell Biology, Cell Culture, Conditioned media supplements and reagents, Folic Acid Metabolism, Hybridoma Reagents,
InChI Key   TVZGACDUOSZQKY-LBPRGKRZSA-N
product line   BioReagent
assay   ≥97%
form   powder
color   yellow
solubility   2 M NaOH: 50 mg/mL
  DMSO: soluble
suitability   suitable for cell culture
storage temp.   −20°C
Gene Information   human ... FPGS(2356)
mouse ... Fpgs(14287)

Description

Application

Aminopterin has been used in the production of anti- neurogranin antibodies.

Biochem/physiol Actions

Aminopterin is found to inhibit growth, embryogenesis and has toxic effects in mice models. It serves as a abortifacient. Aminopterin is known to reduce the effects of acute leukemia in children.

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Linkage

More potent, but more toxic, than methotrexate.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGI
WGK Germany 
3
RTECS 
MA1050000

Documents

Certificate of Analysis

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Certificate of Origin

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Pipette Controller from Brand

Cell Culture Guide
Protocols & Articles

Articles

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aminopterin and Related Compounds on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18
Keywords: Clinical, Pharmaceutical, Separation

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Peer-Reviewed Papers
15

References

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A5159 Aminopterin, Hybri-Max, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
06693 Timestrip Plus -20 °C

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