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  • A3415 - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

A3415 Sigma

Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

Synonym: Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey - Tested Applications: direct ELISA 1:10,000 - Sigma Aldrich A3415, Anti Sheep Antibody, Anti Sheep Hrp



Related Categories Alphabetical Index, Antibodies, Peroxidase Labeled Antibodies, Primary Antibodies, SG-SK,
biological source   donkey
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   sheep
application(s)   direct ELISA: 1:10,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C



Purified sheep IgG


0.5 mL in glass insert

1 mL in glass bottle

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal


Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

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The following information is provided to help you decide which secondary antibody may be best for your particular application.
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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Peer-Reviewed Papers


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