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A3553 Sigma-Aldrich

Acrylamide

for electrophoresis, ≥99% (HPLC), powder

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein Registry Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  eCl@ss 39031205

  •  PubChem Substance ID 24890783

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Properties

Related Categories Acrylamides, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis, Life Science Reagents for Protein Expression and Purification,
grade   for electrophoresis
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
InChI Key   HRPVXLWXLXDGHG-UHFFFAOYSA-N
assay   ≥99% (HPLC)
form   powder
impurities   ≤0.005% Acrylic acid
  ≤0.005% Insoluble in water
conductivity   ≤10 μmho (35% solution)
bp   125 °C/25 mmHg(lit.)
mp   82-86 °C (lit.)
solubility   water: soluble 2.5 g/10 mL, clear, colorless
cation traces   Fe: ≤1 ppm
  Mg: ≤3 ppm
  heavy metals (as Pb): ≤5 ppm

Description

Compatibility

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

Packaging

1 kg in glass bottle

100, 500 g in glass bottle

Application

Acrylamide has been used in the preparation of polyacrylamide gels.

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
3
RTECS 
AS3325000
Flash Point(F) 
280.4 °F
Flash Point(C) 
138 °C

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Protocols & Articles
Peer-Reviewed Papers
15

References

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