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A3678 Sigma-Aldrich

Ammonium persulfate

for molecular biology, for electrophoresis, ≥98%

Synonym: AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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Properties

Related Categories Chemical Synthesis, Core Bioreagents, Electrophoresis of Glycans, Gel Casting Reagents, General Reagents,
Quality Level   200
grade   for electrophoresis
  for molecular biology
vapor density   7.9 (vs air)
assay   ≥98%
anion traces   chloride (Cl-): <10 ppm
cation traces   Fe: <10 ppm
  heavy metals (as Pb): <50 ppm
SMILES string   N.N.OS(=O)(=O)OOS(O)(=O)=O
InChI   1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)
InChI key   ROOXNKNUYICQNP-UHFFFAOYSA-N

Description

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

Packaging

25, 100 g in poly bottle

Analysis Note

Tested for use in acrylamide polymerization.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Respiratory system
RIDADR 
UN 1444 5.1 / PGIII
WGK Germany 
WGK 1
RTECS 
SE0350000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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