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  • A3713 - Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)−Alkaline Phosphatase antibody produced in mouse

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A3713 Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)−Alkaline Phosphatase antibody produced in mouse

clone MAPK-YT, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2)

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Properties

clone   MAPK-YT, monoclonal
application(s)   western blot: 1:250 using rat brain extract
species reactivity   mouse, Xenopus, Drosophila, Caenorhabditis elegans, rat, yeast, hamster, bovine, human
mol wt   antigen ERK-1 44 kDa
  antigen ERK-2 42 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
Gene Information   human ... MAPK1(5594), MAPK3(5595)
mouse ... Mapk1(26413), Mapk3(26417)
rat ... Mapk1(116590), Mapk3(50689)

Description

Specificity

The antibody reacts specifically with the diphosphorylated form of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase.

Immunogen

synthetic peptide HTGFLpTEpYVAT corresponding to the phosphorylated form of the ERK-activation loop.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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