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A3983 Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially γ-globulin free, ≥98% (agarose gel electrophoresis)

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Heat Shock Fractionated, New Zealand Origin Bovine Serum Albumins,
biological source   bovine
assay   ≥98% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   ~66 kDa
purified by   heat shock fractionation
packaging   poly bottle of
origin   New Zealand origin
impurities   essentially globulin free
loss   ≤5%
pH   7
solubility   water: soluble, clear to slightly hazy (1 g + 25 mL)
foreign activity   Protease free
  Protease free
storage temp.   2-8°C

Description

Packaging

10, 50, 100, 500 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Derived from New Zealand source serum

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
MT6446000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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