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  • A4062 - Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

A4062 Sigma

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alkaline Phosphatase, Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibody Conjugates,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
species reactivity   goat
application(s)   direct ELISA: 1:30,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
  western blot: 1:30,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


Other Notes

Antibody adsorbed with human serum proteins.


purified goat IgG

Preparation Note

Adsorbed to reduce background staining with human samples.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide


Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate.

Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) .

General description

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. It can also be used in immunohistochemistry. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins.

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


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