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A4327 Sigma-Aldrich

Albumin from human serum

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

Synonym: HSA

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Properties

Related Categories Albumin, Biochemicals and Reagents, Human Serum Albumins, Proteins and Derivatives
Quality Level   200
biological source   human
assay   ≥96% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   monomer calculated mol wt 66478 Da
application(s)   tissue culture: suitable
impurities   HIV I and HIVII, HCV and HBsAg, tested negative
solubility   H2O: soluble 50 mg/mL
foreign activity   Alkaline phosphatase ≤0.001 units/mg protein
  Peroxidase ≤0.001 units/mg protein
  Protease ≤0.0001 units/mg protein
storage temp.   2-8°C
Gene Information   human ... ALB(213)

Description

General description

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:
• Essentially protease-free / low protease content
• Low alkaline phosphatase content
• Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:
• Enzyme assays
• Protein-based assays
• Protease-sensitive techniques such as:
(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

Application

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

Packaging

1 g in glass bottle

500 mg in glass bottle

Biochem/physiol Actions

Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.

Features and Benefits

• Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
• Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
• Low peroxidase activity (≤ 0.001 units/mg protein)

Preparation Note

Prepared from A1653

Other Notes

View more information on human serum albumin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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A9731 Albumin human, recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)

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