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  • A4351 - Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit

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A4351 Sigma-Aldrich

Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-A Disintegrin And Metalloproteinase-17, Anti-TACE, Anti-TNF-α Converting Enzyme

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Description

General description

ADAM-17 or tumor necrosis factor converting enzyme (TACE) is known to regulate several cellular functions such as cell growth and movement. It has a varied array of substrates that include adhesion molecules, growth factors and their receptors, as well as many cytokines. Thus, ADAM-17 abnormalities have been associated with various disorders such as renal fibrosis, Alzheimer′s disease, rheumatoid arthritis, cancer, diabetes and cardiac disorders.
Rabbit anti-ADAM-17, cytoplasmic domain antibody recognizes human, porcine, and rat ADAM17. It does not react with other ADAMs. By immunoblotting against the reduced protein, the antibody recognizes a band of 110kDa from conditioned media or cell lysates.

Immunogen

synthetic peptide corresponding to the cytoplasmic domain of full-length human ADAM-17.

Application

Rabbit anti-ADAM-17, cytoplasmic domain antibody can be used for western blot at a dilution of 1:1,000.

Physical form

Solution in 0.01 M phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
1

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
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References

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