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A4352 Sigma-Aldrich

Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer831) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-phospho-AMPA Glutamate Receptor 1 (pSer831)

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Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   direct ELISA: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: 1:1,000 using rat brain (hippocampal) homogenate
species reactivity   rat, human
mol wt   antigen mol wt 100 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P42261
Gene Information   human ... GRIA1(2890)
rat ... Gria1(50592)

Description

General description

AMPA is a transmembrane receptor for glutamate that regulates synapsis in the central nervous system. It is composed of 4 types of subunits, GluR1, 2, 3, 4.

GluR1 subunits of AMPA receptors are membrane proteins that regulate synaptic plasticity of the central nervous system. This receptor has also been implicated in spatial memory retention. Phosphorylation of serine 831 in GluR1 subunit of the AMPA receptor modulates long-term potentiation in neuronal synapses. Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer831) antibody is specific for the GluR1 subunit (phosphorylated on serine 831) of the AMPA receptor in rats and humans.

Immunogen

The GluR1 sequence is conserved among species and has partial homology to VGLUT2.

synthetic phosphopeptide corresponding to a region near serine 831 of rat glutamate receptor subunit GluR1.

Application

Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer831) antibody is suitable for use in direct ELISA, immunohistochemistry, immunoblot and immunoprecipitation. The antibody may also be used for western blot (1:1,000 using rat hippocampal homogenate).

Physical form

Solution in 10 mM HEPES buffer, pH 7.5, containing 150 mM NaCl, 100 μg/ml BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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