EMAIL THIS PAGE TO A FRIEND
A4403 Sigma-Aldrich

L-Ascorbic acid

powder, suitable for cell culture, γ-irradiated

Synonym: L-Threoascorbic acid, Antiscorbutic factor, Vitamin C

  • CAS Number 50-81-7

  • Empirical Formula (Hill Notation) C6H8O6

  • Molecular Weight 176.12

  •  Beilstein/REAXYS Number 84272

  •  EC Number 200-066-2

  •  MDL number MFCD00064328

  •  PubChem Substance ID 24890857

  •  NACRES NA.75

Purchase

Properties

Related Categories Amino Acids, Amino Acids & Vitamins, Amino Acids and Vitamins, Biochemicals and Reagents, Cell Culture,
Quality Level   200
sterility   γ-irradiated
form   powder
mol wt   Mw 176.12 g/mol
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
  cell culture | plant: suitable
color   white to off-white
pH   1.0-2.5 (25 °C, 176 g/L in water)
mp   190-194 °C (dec.)
solubility   H2O: 10 mg/mL
SMILES string   OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O
InChI   1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1
InChI key   CIWBSHSKHKDKBQ-JLAZNSOCSA-N
Gene Information   human ... SLC23A2(9962)

Description

General description

L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.

Application

For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
• in an osteogenic supplement for the growth of cells
• as a component in the differentiation media
• in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects

Biochem/physiol Actions

L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
CI7650000

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Related Content

Ascorbate in Cell Culture

Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Cell culture, Culture media, Hydroxylations, Lipid peroxidation, Metabolism, Peroxidations, Vitamins

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Life Science Materials

With a broad portfolio of widely-cited reagents and kits supported by industry-leading expertise, we help you get the most out of your life science research.

Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Add to Cart

CLS2592 Corning® 1 x 8 Stripwell 96 well plates, high binding surface, clear

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?