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A4464 Sigma

Enhanced Avian Reverse Transcriptase [eAMV RT]

For reverse transcription at higher temperatures & rare mRNAs



Features and Benefits

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb


Provided with a vial of 10× reaction buffer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

eAMV is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.


Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Safety & Documentation
Protocols & Articles


Reverse Transcription - PCR Technologies Guide

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in halflife between different...
Keywords: Degradations, Detection methods, Gene expression, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

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RT-PCR Fundamentals

Reverse transcriptase PCR, or RT-PCR, is a variation of standard PCR technique, which involves the amplification of specific mRNA obtained from very small samples. It eliminates the need for the tedi...
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Peer-Reviewed Papers


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