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A4464 Sigma

Enhanced Avian Reverse Transcriptase [eAMV RT]

For reverse transcription at higher temperatures & rare mRNAs

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Description

Features and Benefits

• Greater sensitivity for low abundance mRNA
• Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
• Efficient generation of full-length cDNA, up to 14.1 kb

Packaging

Provided with a vial of 10× reaction buffer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

eAMV is a trademark of Sigma-Aldrich Co. LLC

Unit Definition

One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.

Application

Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.

Biochem/physiol Actions

Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.

Price and Availability


PCR Selection Tool

Kapa Biosystems

Biomedical Applications
Safety & Documentation
Protocols & Articles

Articles

Reverse Transcription - PCR Technologies Guide

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in halflife between different...
Keywords: Degradations, Detection methods, Gene expression, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

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PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
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RT-PCR Fundamentals

Reverse transcriptase PCR, or RT-PCR, is a variation of standard PCR technique, which involves the amplification of specific mRNA obtained from very small samples. It eliminates the need for the tedi...
Keywords: Amplification, Cloning, Diagnostic, Microbiology, Molecular biology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription, Whole genome amplification

Peer-Reviewed Papers
15

References

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