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A4477 Sigma-Aldrich

Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer845) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-AMPA Glutamate Receptor 1 (pSer845)

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Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:1000
  western blot: 1:1,000 using rat brain extracts
species reactivity   rat, human
mol wt   antigen mol wt 100 kDa
form   buffered aqueous glycerol solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P42261
Gene Information   human ... GRIA1(2890)
rat ... Gria1(50592)

Description

General description

Rabbit Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer845) antibody recognizes human and rat AMPA Receptor, GluR1 subunit (pSer845).

Immunogen

synthetic phosphorylated peptide corresponding to a region near serine 845 of rat glutamate receptor subunit GluR1.

Application

Rabbit Anti-phospho-AMPA Receptor, GluR1 Subunit (pSer845) antibody can be used for western blot analysis at 1:1,000 using rat brain extracts.

Target description

AMPA is a transmembrane receptor for glutamate that regulates synapsis in the central nervous system. It is composed of 4 types of subunits, GluR1, 2, 3, 4.

Physical form

Solution in 10 mM HEPES buffer, pH 7.5, containing 150 mM NaCl, 100 μg/ml BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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