• USA Home
  • A4541 - Anti-Biotin−Peroxidase antibody produced in goat

A4541 Sigma

Anti-Biotin−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym: Anti Biotin Hrp, Anti-Biotin



Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:2,000
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C



Biotin-KLH conjugate


0.25, 0.5 mL in glass insert

1 mL in glass bottle

Preparation Note

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal


Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.

Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

General description

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Price and Availability

Antibody Explorer

Explore with Confidence

Celebrate Pi Day!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?