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A4551 Sigma-Aldrich

α-Amylase from Bacillus licheniformis

Green Alternative

lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE)

Synonym: 1,4-α-D-Glucan-glucanohydrolase




1 g in glass bottle

100 mg in glass bottle

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing potassium phosphate

General description

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What information is available regarding the solubility of Product A4551, alpha-Amylase from Bacillus licheniformis?
Product A4551 is a lyophilized alpha-amylase. Although it is reasonable to expect it to dissolve in water or dilute buffer, we do not have a solubility specification; see current specifications on our website. When we measure the specific activity of this enzyme, we typically make our first dilution in cold purified (deionized) water at about 2-3 mg/mL, followed by an intermediate dilution into purified water, followed by dilution into the reaction buffer to start the reaction.When making a solution of the enzyme, it is OK to stir gently with a magnetic stir bar; do not vortex or sonicate.
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Protocols & Articles


Enzymatic Assay of α-Amylase (EC

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

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