• USA Home
  • A4594 - Anti-ADP Ribosylation Factor 1/3 (ARF 1/3) antibody produced in sheep

A4594 Sigma

Anti-ADP Ribosylation Factor 1/3 (ARF 1/3) antibody produced in sheep

IgG fraction of antiserum, buffered aqueous solution



Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to ARF and Related Proteins,
species reactivity   human, mouse
application(s)   western blot: 1:500-1:2,000 using RIPA lysates of mouse brain cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 21 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... ARF1(375), ARF3(377)
mouse ... Arf1(11840), Arf3(11842)
biological source   sheep
conjugate   unconjugated



peptide corresponding to amino acids 98-112. Selected for maximal sequence homology with human ARF 1 and 3, but minimal sequence homology to other known ARFs.

Physical form

Solution in glycine buffered saline, containing 0.1% EACA, 0.01% benzamidine, 1 mM EDTA, and 0.1% sodium azide.

Preparation Note

The antibody is supplied as a DEAE purified IgG fraction.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?