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A4675 Sigma-Aldrich

Agar

Type E, suitable for plant cell culture

Synonym: Agar-agar, Gum agar

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Core Bioreagents, Culture Media Base Ingredient - Agar, Gelling Agents,
Quality Level   200
biological source   algae (Rhodophyceae)
type   Type E
form   powder
application(s)   cell culture | plant: suitable
transition temp   transition temp 32-38 °C (1.5%)
Featured Industry   Agriculture
storage temp.   room temp

Description

General description

Agar, an algal polysaccharide, has gel forming properties. Structure of agar has been investigated. Growth of human bone marrow cell colonies in agar-gel medium have been described.

Application

Agar was used as reagent for the following studies:
• Plant cell culture studies.
• As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
• For the solidification of culture medium of cork oak somatic embryos.
It may be used for incubation of single antibody-forming cell to investigate the plaque formation. Agar suspension culture may be used for the analysis of cell transformation of palyoma virus.

Packaging

1 kg in poly bottle

500 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
RTECS 
AW7950000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Gelling Agents - Plant Tissue Culture Protocol

Agar has long been used to solidify media for plant tissue culture. The type of agar or gelling agent used can influence the growth of the tissue in culture. Both purity and cost of the gelling agent...
Keywords: Cell culture, Centrifugation, Immobilization, Inductively coupled plasma, Reductions

Peer-Reviewed Papers
15

References

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