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A4941 Sigma-Aldrich

Activin A human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, lyophilized powder, cell culture tested

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Properties

Related Categories Activins, Activins and Inhibins, Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology,
biological source   human
recombinant   expressed in baculovirus infected insect cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   1-10 ng/mL ED50
mol wt   dimer 26 kDa (containing 116 amino acid residues βA subunits)
packaging   pkg of 10 μg
  pkg of 5 μg
impurities   endotoxin, tested
suitability   cell culture tested
UniProt accession no.   P08476
storage temp.   −20°C
Gene Information   human ... INHBA(3624)

Description

Biochem/physiol Actions

Activins are classified as members of the TGF-β superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-β forms; regulate hormonogenesis, cellular homeostasis and differentiation programs.

Physical form

Lyophilized from 0.2 μm filtered solution in 10 mM sodium citrate, pH 3.0.

Analysis Note

The biological activity is determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

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