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A5043 Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Synonym: Adenosine aminohydrolase



Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type X
form   buffered aqueous glycerol solution
specific activity   ≥130 units/mg protein
mol wt   32.5-33 kDa
cation traces   NH4+: ≤10 μg/mg protein
foreign activity   alkaline phosphatase ≤0.01%
  guanase ≤0.01%
  nucleoside phosphorylase ≤0.1%
storage temp.   2-8°C
Gene Information   cow ... ADA(280712)


General description

Molecular weight: 32.5-33 kDa

pI: 4.85


Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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