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  • A5101 - Anti-ADAM-9, Propeptide Domain antibody produced in rabbit

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A5101 Sigma-Aldrich

Anti-ADAM-9, Propeptide Domain antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-A Disintegrin And Metalloproteinase-9, Anti-MDC9, Anti-Meltrin-γ

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000
species reactivity   pig, rat, human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q13443
Gene Information   human ... ADAM9(8754)
rat ... Adam9(290834)

Description

General description

ADAM9 (MDC9 or Meltrin-gamma) belongs to the metalloproteinase family that has disintegrin-like domains (ADAMs). ADAM9 is widely expressed in tissues such as heart, lung and brain cells. This metalloproteinase regulates myoblast functions and activates TNF-α (tumor necrosis factor α) and HB-EGF. ADAM9 also functions as an adhesion protein with integrins αvβ5 and α6β1.
Anti-ADAM-9, Propeptide Domain may be used for the detection and localization of human ADAM9 and does not react with other ADAMs. The antibody is also reacts with rat, pig and human ADAM9.

Recognizes bands at 116 kDa (minor) and 84 kDa (major), and breakdown products at 50 kDa and 34 kDa in reduced immunoblots.

Immunogen

synthetic peptide corresponding to the propeptide domain of human ADAM-9.

Application

Rabbit Anti-ADAM-9, Propeptide Domain antibody can be used for western blot (1:1,000) applications.

Physical form

Solution in 0.01 M phophate buffered saline containing 50% glycerol and 0.05% sodium azide.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
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References

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