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  • A5228 - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal

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A5228 Sigma-Aldrich

Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal

clone 1A4, purified from hybridoma cell culture

Synonym: Acta2 Antibody, Alpha Smooth Muscle Actin Antibody Sigma, Monoclonal Anti-Actin, α-Smooth Muscle, SMA Antibody - Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, SMA, Sma Antibody

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Properties

Related Categories AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology,
conjugate   unconjugated
clone   1A4, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable using smooth muscle cells
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
  indirect ELISA: suitable
  indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections
  microarray: suitable
  western blot: suitable using smooth muscle cells
species reactivity   rabbit, guinea pig, mouse, chicken, snake, sheep, goat, human, frog, rat, canine, bovine
mol wt   antigen mol wt ~42 kDa
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
Featured Industry   Research Pathology
UniProt accession no.   P62736
Gene Information   human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Description

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Application

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Mouse Monoclonal Anti-Actin, α-Smooth Muscle antibody can be used for whole-mount immunohistochemistry and immunofluorescence studies. The product can also be used for microarray and western blot analyses.

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Packaging

200 μL in glass bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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