EMAIL THIS PAGE TO A FRIEND
A5354 Sigma-Aldrich

Ampicillin

Ready Made Solution, 100 mg/mL, 0.2 μm filtered

Purchase

Properties

Related Categories A - K, Antibacterial, Antibiotic Ready Made Solutions, Antibiotics, Antibiotics A to Z,
biological source   microbial
sterility   0.2 μm filtered
form   liquid
autoignition temp.   301 °C
concentration   100 mg/mL
suitability   suitable (for antibacterial use in culture media)
Mode of action   cell wall synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C

Description

Biochem/physiol Actions

A β-lactam antibiotic with an amino group side chain attached to the penicillin structure. Penicillin derivative that inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Bactericidal only to growing Escherichia coli. Mode of resistance: Cleavage of β-lactam ring of ampicillin by β-lactamase. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.

Application

Ampicillin is used to select bacteria cells with specific resistance during general microbiology studies. It is used to study antibiotic resistance and penetration limitations, the synergy between multiple antibiotics, certain bloodstream infections, and has been used to develop PCR assays to detect resistance genes in cerebrospinal fluid .

General description

Ampicillin in a convenient ready-to-use solution useful in selecting for bacteria cells with specific resistance.

Chemical structure: ß-lactam

Other Notes

Add your comments, questions, and reviews about this product.

See what your peers are saying about this product.

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefullyresealed and kept upright to prevent leakage.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
WGK Germany 
2
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

A transcription activator-like effector toolbox for genome engineering

Neville E Sanjana a, b , Le Cong a, b, c , Yang Zhou a, b , Margaret M Cunniff a, b , Guoping Feng a, b , Feng Zhang a, b
Keywords: Peptide synthesis, Polymerase chain reaction, Transcription

Genome engineering using the CRISPR-Cas9 system

F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Scott a, b, c, d , Feng Zhang a, b, c, d
Keywords: Amplification, Antibiotics, Antivirals, Apoptosis, Catalysis, Cell culture, Cell dissociation, Central Nervous System, Chromatin immunoprecipitation, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Genetics, Growth factors, Melting, Nucleic acid annealing, PAGE, Peptide synthesis, Polymerase chain reaction, Polymorphisms, Purification, Rearrangements, Recombination, Sample preparations, Sequencing, Transcription, Transfection, transformation

Related Content

Ampicillin (A5354) Discussion and Reviews

If you would like to leave a review, question, application info, or other comment, follow this Link.

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?