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  • A5420 - Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

A5420 Sigma

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti Goat Igg, Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit - Tested Applications: immunohistochemistry (formalin-fixed, paraffin-embedded sections) 1:200,direct ELISA 1:40,000,dot blot 1:80,000-1:160,000 - Sigma Aldrich A5420



Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Antibody-Peroxidase Conjugates, GM-GO,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   goat
application(s)   direct ELISA: 1:40,000
  dot blot: 1:80,000-1:160,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C



purified goat IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal


LNCAP cell lysates were analyzed by western blot using HRP conjugated rabbit anti-goat IgG as the secondary at a 1:7500 dilution in TBSt for 1 hour at room temperature.

Rabbit Anti-Goat IgG (whole molecule)-Peroxidase antibody has been used for immunoblotting assays. The antibody can also be used for direct ELISA (1:40,000), dot blot (1:80,000-1:160,000) and immunohistochemistry (1:200) assays.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody binds to all goat Igs.

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