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A5503 Sigma

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym: Albumin from hen egg white, Ovalbumin

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Properties

Related Categories Albumin, Albumins from other species, Biochemicals and Reagents, Control Proteins for Glycosylation Studies, Glycobiology,
Quality Level   PREMIUM
type   Grade V
assay   ≥98% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   mol wt 44287 Da by calculation
purified by   crystallization
solubility   H2O: soluble 40 mg/mL
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Albumin is useful as a marker (∼45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma. Albumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.
Studies to test the effects of localization on DNA immunizations use albumin in antibody assays.

Packaging

1, 5, 10, 25, 50 g in poly bottle

Physical form

Essentially salt free

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AY9384000

Frequently Asked Questions

Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?
A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.
What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?
We have only tested solubility in water. Salts in buffers may make solubility more difficult. 
How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?  
A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE. 
Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8 deg C. Does it ship on ice?
A5503 does not require shipping on ice.
Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?
We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A400 Protein C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A400 Protein C18
Keywords: High performance liquid chromatography

HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles
Keywords: Antibiotics, Antimicrobials, Chromatography, Clinical, High performance liquid chromatography, Separation, Size-exclusion chromatography

Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume

From our library of Articles, Sigma-Aldrich presents Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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