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A5503 Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym: Albumin from hen egg white, Ovalbumin

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Properties

Related Categories Albumin, Albumins from other species, Biochemicals and Reagents, Control Proteins for Glycosylation Studies, Glycobiology,
Quality Level   PREMIUM
biological source   chicken egg white
type   Grade V
assay   ≥98% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   44287 Da by calculation
purified by   crystallization
solubility   H2O: soluble 40 mg/mL
storage temp.   2-8°C

Description

General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.

Application

Albumin is useful as a marker (∼45 kDa) for protein electrophoresis. It has been used to produce an animal model of asthma. Albumin was used as medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil.
Studies to test the effects of localization on DNA immunizations use albumin in antibody assays.

A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Packaging

1, 5, 10, 25, 50 g in poly bottle

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

Physical form

Essentially salt free

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AY9384000

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?
A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.
What is the solubility of Product No.A5503, Albumin from chicken egg white,in aqueous buffers (e.g. Tris, PBS)?
We have only tested solubility in water. Salts in buffers may make solubility more difficult.
How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?
A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.
Product No. A5503, Albumin from chicken egg white,is to be stored at 2 - 8 deg C. Does it ship on ice?
A5503 does not require shipping on ice.
Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?
We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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