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A5633 Sigma-Aldrich

Antifoam A Concentrate

active silicone polymer 100 %

  •  NACRES NA.25

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Properties

Related Categories Anti-Foaming Agents, Antifoams, Biochemicals and Reagents, Detergents, Detergents A to M,
Quality Level   200
description   100% active silicone polymer
does not contain   emulsifier
composition   active silicone polymer, 100%
application(s)   cell culture | mammalian: suitable
  microbiological culture: suitable

Description

General description

Antifoam A Concentrate is an extremely effective foam suppressor for aqueous and non-aqueous systems. It is 100% active silicone polymer. No emulsifiers are present. Antifoam A Concentrate is typically effective at 1-100 ppm. Antifoam A Concentrate should be diluted with 3-10 parts of propylene glycol (aqueous) or vegetable oil (nonaqueous) with slow mixing. The product will be stable in the pH range of 5 to 9. Antifoam A Concentrate can be added directly to a fermentation medium, but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Application

An extremely effective foam suppresser for use in aqueous and non-aqueous systems.

Antifoam A Concentrate has been used as a component of Luria Bertani culture medium for growth of BL21 (DE3) E. coli cells transformed with the plasmids. It has also been used as a constituent in the preparation of aerosol generation fluid.

Packaging

100 g in poly bottle

25 g in glass bottle

Reconstitution

1-50 ppm is adequate for most applications.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antifoams

A6426 Antifoam 204 A8311 Antifoam 204 (autoclaved) A5633 Antifoam A Concentrate A6582 Antifoam A Concentrate Molecular biology grade A5757 Antifoam B Emulsion A8011 Antifoam C Emulsion A5758 Antifoam...
Keywords: Antimicrobials, Culture media, Fermentation, Filtration, Molecular biology, Nucleic acid denaturation, Sample preparations, Separation

Peer-Reviewed Papers
15

References

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