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A5691 Sigma-Aldrich

Monoclonal Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Actin, α-Smooth Muscle, SMA

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology,
conjugate   alkaline phosphatase conjugate
clone   1A4, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
  western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
species reactivity   human, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake
mol wt   antigen ~42 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
UniProt accession no.   P62736
Gene Information   mouse ... Acta2(11475)
rat ... Acta2(81633)

Description

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibodies to Actin Gene Family Isoforms

The Actin Gene Family Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular p...
Keywords: Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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