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A5754 Sigma-Aldrich

3-Amino-9-ethylcarbazole

≥95% (HPLC), powder

Synonym: 9-Ethylcarbazol-3-amine, AEC

  • CAS Number 132-32-1

  • Empirical Formula (Hill Notation) C14H14N2

  • Molecular Weight 210.27

  •  Beilstein/REAXYS Number 174969

  •  MDL number MFCD00004964

  •  PubChem Substance ID 24891017

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Description

General description

3-Amino-9-ethylcarbazole serves as chromogen in immunocytochemistry and produces a red color.

Specificity

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

Application

3-Amino-9-ethylcarbazole has been used to visualize the bound primary antibody by using a streptavidin method. It has also been used in visualizing tissue sections from tumour samples.

Packaging

10, 50, 100 g in glass bottle

Substrates

Substrate for peroxidase.

Analysis Note

Actual content given on label

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

Add to Cart

AEC101 AEC Staining Kit, liquid
A6926 3-Amino-9-ethylcarbazole, tablet

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