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  • A5838 - Anti-Glutathione-S-Transferase (GST)–Alkaline Phosphatase Conjugate antibody produced in rabbit

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A5838 Sigma-Aldrich

Anti-Glutathione-S-Transferase (GST)–Alkaline Phosphatase Conjugate antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Glutathione-S-Transferase, Anti-GST

  •  NACRES NA.46

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Properties

Related Categories Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags,
conjugate   alkaline phosphatase conjugate
clone   polyclonal
biological source   rabbit
application(s)   direct ELISA: 1:400 using using wells coated with 5 μg/mL GST in carbonate buffer
  western blot (chemiluminescent): 1:20,000
mol wt   antigen 27.5 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies

Description

General description

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Alkaline phosphatase (AP) conjugated anti-GST antibodies have been used as probes for western blot assays. The product can also be used for direct ELISA (1:400).

Purification of GST-SfMannIII from Sf9 insect cells was confirmed by western blot using rabbit anti-GST antibody. Gst-SfMan I was purified from over-expressing SF/SWT-1 cells and ran on SDS-page fell for western blot analysis using rabbit anti-GST as the primary antibody. Alkaline phosphatase-conjugated goat anti-rabbit IgG (Sigma) was used as the secondary antibody.

Physical form

Solution supplied in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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