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A6106 Sigma-Aldrich

Aprotinin from bovine lung

BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

Synonym: Trasylol, Trypsin inhibitor (basic)

  • CAS Number 9087-70-1

  • Empirical Formula (Hill Notation) C284H432N84O79S7

  • Molecular Weight 6511.44

  •  EC Number 232-994-9

  •  MDL number MFCD00130541

  •  NACRES NA.77

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Properties

Related Categories Biochemicals and Reagents, Enzyme Inhibitors, Enzyme Inhibitors by Type, Enzymes, Inhibitors, and Substrates, Proteins More...
Quality Level   300
product line   BioUltra
assay   ≥98% (SDS-PAGE)
form   powder
mol wt   ~6,500
solubility   H2O: 5 mg/mL, clear to very slightly hazy
storage temp.   2-8°C

Description

Packaging

1 mg in glass bottle

25, 100 mg in poly bottle

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Preparation Note

A further purification of A1153 to yield a product with slightly higher activity and purity

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YN5080000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Aprotinin

The scope of this procedure is all products that have a specification for Aprotinin activity.
Keywords: Enzymology, Extinction coefficient

Peer-Reviewed Papers
15

References

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