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A6270 Sigma-Aldrich

Azure A chloride

Dye content ≥70 %

  • CAS Number 531-53-3

  • Empirical Formula (Hill Notation) C14H14ClN3S

  • Molecular Weight 291.80

  •  Colour Index Number 52005

  •  Beilstein/REAXYS Number 3922287

  •  EC Number 208-510-7

  •  MDL number MFCD00012112

  •  PubChem Substance ID 24891073

  •  NACRES NA.47

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Properties

Related Categories A, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
form   powder or crystals
composition   Dye content, ≥70%
application(s)   hematology: suitable
  histology: suitable
mp   290 °C (dec.) (lit.)
solubility   water: 1 mg/mL
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI   1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key   NALREUIWICQLPS-UHFFFAOYSA-N

Description

Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.

Packaging

10, 50 g in glass bottle

Biochem/physiol Actions

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Caution

Handle and store under argon. Glove bag item.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SP5660000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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