• USA Home
  • A6354 - Anti-Adiponectin antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
A6354 Sigma-Aldrich

Anti-Adiponectin antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Adiponectin Antibody, Anti-Adipocyte Complement-Related Protein of 30 kDa, Anti-Adiponectin antibody produced in rabbit

Purchase

Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Mesenchymal Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot (chemiluminescent): 0.5-1 μg/mL using using human plasma or placenta, or an extract of differentiated 3T3-L1 mouse cells. (It is recommended to dilute the antibody in phosphate buffered saline containing 0.5% non-fat dry milk for background staining reduction.)
species reactivity   human, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   Q15848
Gene Information   human ... ADIPOQ(9370)
mouse ... Adipoq(11450)

Description

General description

Adiponectin is secreted from adipose tissue for the regulation of glucose and fatty acid break down.

Anti-Adiponectin is produced in rabbit using a synthetic peptide corresponding to amino acid residues of human adiponectin. Adiponectin, also designated as adipocyte complement-related protein of 30 kDa (Acrp30), AdipoQ, apM1, Gelatin-binding protein 28 kDa (GBP28) is an adipocyte-specific protein that circulates in the blood stream, where it accounts for approximately 0.01% of all plasma proteins. Adiponectin belongs to the complement factor C1q family that belongs to the soluble defense collagen superfamily. Adiponectin (ADIPOQ) protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain.

Specificity

Rabbit Anti-Adiponectin antibody recognizes human and mouse adiponectin.

Immunogen

synthetic peptide corresponding to amino acid residues 225-244 of human adiponectin with N-terminal added cysteine, conjugated to KLH.

Application

Anti-Adiponectin antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit Anti-Adiponectin antibody has been used for immunohistochemical and western blot applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Adipose tissue has important roles in energy storage, glucose homeostasis and fat metabolism.(10) Increased adiponectin levels are associated with type I diabetes mellitus, anorexia nervosa, weight reduction, and chronic renal failure. Adiponectin (ADIPOQ) affects lipid catabolism by increasing fatty acid oxidation in muscle, modulates glucose uptake, alters liver gluconeogenesis and enhances insulin-sensitivity in both muscle and liver. ADIPOQ has anti-atherosclerotic and anti-inflammatory properties. ADIPOQ has a role in physiological processes such as energy homeostasis and obesity. Low levels of adiponectin are also linked with hypertension and dyslipidemia. Mutation in the gene encoding it is linked with risk for endometrial cancer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?