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A6380 Sigma-Aldrich

α-Amylase from Bacillus sp.

Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

Synonym: 1,4-α-D-Glucan-glucanohydrolase

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Description

Packaging

1 g in glass bottle

100, 250, 500 mg in glass bottle

25 mg in poly bottle

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Preparation Note

4× crystallized

Dissolves in water to form a clear, colorless solution at 0.1 mg/mL concentration.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
BU7432500
Protocols & Articles

Protocols

Enzymatic Assay of Alpha-Amylase

The spectrophotometric stop reaction determination (A540, Light path = 1 cm) is based on the following reaction:

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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