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  • A6477 - Anti-ADAMTS-3, C-Terminal antibody produced in rabbit

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A6477 Sigma-Aldrich

Anti-ADAMTS-3, C-Terminal antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-A Disintegrin And Metalloproteinase with ThromboSpondin-3 motif

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000
species reactivity   human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   O15072
Gene Information   human ... ADAMTS3(9508)

Description

General description

ADAMTS-3 belongs to the ADAM (A Disintegrin And Metalloproteinase) family and has thrombospondin (TS) repeats. Studies have suggested that ADAMTS-3 is preferably expressed in the cartilages and has procollagen II N-propeptidase functions. Rabbit Anti-ADAMTS-3, C-Terminal antibody binds to the C-terminus of human ADAMTS-3.

By immunoblotting against the reduced protein, the antibody identifies the zymogen form at 130-140 kDa, activated forms at 94-105 kDa (major bands), and breakdown products at 50 kDa, 34 kDa in cell culture media and lysates.

Immunogen

synthetic peptide corresponding to the C-terminus of human ADAMTS-3.

Application

Rabbit Anti-ADAMTS-3, C-Terminal antibody can be used for western blot applications at a dilution of 1:1,000.

Physical form

Solution in phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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