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  • A6656 - Anti-Human IgG (γ-chain specific)−Agarose antibody produced in goat

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A6656 Sigma-Aldrich

Anti-Human IgG (γ-chain specific)−Agarose antibody produced in goat

IgG fraction of antiserum, saline suspension

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Properties

Related Categories Agarose, Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,
conjugate   agarose conjugate
clone   polyclonal
biological source   goat
application(s)   Ouchterlony double diffusion: suitable
species reactivity   human
form   saline suspension
storage temp.   2-8°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   secondary antibodies
extent of labeling   ≥5 mg per mL
capacity   0.6 mg/mL binding capacity

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat anti-human IgG (γ-chain specific)-agarose antibody is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.

Immunogen

human IgG

Application

Goat anti-human IgG (γ-chain specific)-agarose antibody has been used for immunocapture assays. It can also be used for Ouchterlony double diffusion assays.

IgGs were depleted from normal human serum by incubation with 50 μl of agarose-linked rabbit anti-human antibody (A3543) for 90 minutes at 4°C.

Physical form

Suspension in 0.5 M NaCl and 0.01% thimerosal.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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