• USA Home
  • A6805 - β-N-Acetylglucosaminidase from Streptococcus pneumoniae

EMAIL THIS PAGE TO A FRIEND
A6805 Sigma-Aldrich

β-N-Acetylglucosaminidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

Synonym: β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase

Purchase

Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Antibody Characterization and Analysis, Antibody Glycan Analysis, Application Index,
Quality Level   100
recombinant   expressed in E. coli
form   buffered aqueous solution
activity   ≥80 units/mg protein
packaging   vial of ≥1.0 unit
foreign activity   β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
shipped in   wet ice
storage temp.   2-8°C
Gene Information   Streptococcus pneumoniae R6 ... lytB(934406)

Description

Application

β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.

Biochem/physiol Actions

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

A0500 Acetamide, ~99% (GC)

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

substrate

Product #

Description

Add to Cart

M2133 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide, β-N-acetylhexosaminidase substrate
N9376 4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)
N4006 Naphthol AS-BI N-acetyl-β-D-glucosaminide, β-hexosaminidase substrate

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?