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A6861 Sigma-Aldrich

Acetyl-CoA carboxylase 2 human

recombinant, expressed in Sf9 cells

Synonym: ACACB, ACC2, acetyl-CoA carboxylase beta

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Description

Application

Acetyl-CoA carboxylase is responsible for synthesis of Malonyl-CoA which is an inhibitor of fatty acid oxidation in skeletal muscle mitochondria. The enzyme may be used to study the effect on production of malonyl-CoA as well as fatty acid oxidation during exercise. The enzyme also may be used for ACC regulation study in anti-obesity and anti-type 2 diabetes therapeutics.

Biochem/physiol Actions

Acetyl-CoA Carboxylase (ACC) regulates the metabolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation.

The Acetyl-CoA Carboxylase enzymes are activated by citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Because of thier roles in fatty acid metabolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and metabolic syndrome disorders.

Physical properties

C-terminal HIS-tagged 277 kDa full length protein

Unit Definition

One unit will cause the carboxylation of 1 picomole of acetyl-CoA per minute at pH 7.4 at 30 deg C.

Physical form

Buffered aqueous solution containing Tris-HCl, pH 8.0, NaCl, glycerol, and DTT. May also contain one or more of the following: EDTA, KCl, imidazole, TWEEN-20.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Mitochondrial Dysfunction and Metabolic Defects

Defects in mitochondrial function are associated with insulin resistance in both human and animal studies. Insulin resistance is associated with a decrease in the number, oxidative capacity, size, an...
Keywords: Acetylations, Apoptosis, Biofiles, Cancer, Carboxylations, Catalog, Catalysis, Citric Acid Cycle, Decarboxylations, Degradations, Diabetes, Enzyme-linked immunosorbent assay, Glycolysis, Immunofluorescence, Immunohistochemistry, Lipid Metabolism, Metabolism, Metabolites, Neurotransmitters, Obesity, Oxidations, Phosphorylations, Physiological Processes, Protocols, Transcription, Transduction, Transfection, Western blot

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Peer-Reviewed Papers
15

References

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