• USA Home
  • A6982 - HydroMatrix Peptide Cell Culture Scaffold

EMAIL THIS PAGE TO A FRIEND
A6982 Sigma-Aldrich

HydroMatrix Peptide Cell Culture Scaffold

mixture, powder

Purchase

Description

Components

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

Application

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

Caution

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.

Preparation Note

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

Legal Information

HydroMatrix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What volume of H2O should be used for reconstitution of Product A6982, HydroMatrix Peptide Cell Culture Scaffold?
The package size of the A6982 indicates the volume of H2O to use for reconstitution.For example, a 1 ml package will require 1 ml ofH2O for reconstitution.
WillProduct A6982, HydroMatrix Peptide Cell Culture Scaffold, work will for adherent cells and suspension cells?
Yes, the product works with both cell types.
What is the concentration ofProduct A6982, HydroMatrix Peptide Cell Culture Scaffold, when reconstituted as indicated?
A 1% (w/v) (10 mg/ml) stock solution is accomplished by the addition of sterile dH20 as indicated.
What concentration ofHydroMatrix Peptide Scaffold, Product A6982, should I use for my cells?
A 1% (w/v) solution results in a stronger gel. A working concentration of 0.5% (w/v) results in a soft gel with weak mechanical strength that is suitable for cell migration and angiogenesis.Working solution concentrations as low as 0.150.25% are suitable for many cell types, including neurons. It is recommended for most applications to start with a 0.25% (w/v) working solution (2.5 mg/ml).
How do I embed or encapsulate cells inHydroMatrix Peptide Scaffold, Product A6982, before polymerization?
In a solution of dH20, HydroMatrix has a pH of 2.0-2.5; therefore it must not be added directly to cells.The addition of buffer or media is performed prior to the addition of cells.If performing cell encapsulation studies, addition of iso-osmotic sucrose will help protect the cells until a physiological pH is obtained.
How doesHydroMatrix Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?
Both products are peptide hydrogels.There is no similarity between the peptides used to create the matrices.They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Neural Stem Cell Culture

Neural Stem Cell Lines Neural Stem Cell Media and Supplements Neural Stem Cell Markers Stem Cell Culture Protocols
Keywords: Cell biology, Cell culture, Central Nervous System, Cryopreservation, Diseases, Dopamine agents, Environmental, Gene expression, Growth factors, Neuroscience, Phase transitions, Stem cell biology, Transcription

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?