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A7674 Sigma-Aldrich

Anti-AOP1 antibody, Mouse monoclonal

clone AOP-38, purified from hybridoma cell culture

Synonym: Anti-Antioxidant-like Protein 1, Anti-PRDX3, Anti-Peroxiredoxin 3

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress,
conjugate   unconjugated
clone   AOP-38, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  microarray: suitable
  western blot: 0.25-0.5 μg/mL using a whole extract of cultured human acute T cell leukemia, Jurkat, cells
species reactivity   human, rat, monkey, canine, hamster, mouse, chicken
mol wt   antigen 25 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P30048
Gene Information   human ... PRDX3(10935)
mouse ... Prdx3(11757)
rat ... Prdx3(64371)

Description

General description

Anti-AOP1 (antioxidant-like protein 1) antibody, mouse monoclonal, (antioxidantlike protein 1) (mouse IgG1 isotype) is derived from the AOP-38 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Aop1, conjugated to keyhole limpet hemocyanine (KLH). Aop1 (antioxidant-like protein 1, also called MER5, M.W. 25 kDa).

Antioxidant-like Protein 1 (AOP1) belongs to a protein family that may regulate oxidative stress defense.

Immunogen

synthetic peptide corresponding to C-terminal amino acids 236-256 of human Aop1, conjugated to KLH.

Application

AOP1 expression in cells isolated from human saliva was analyzed by western blot using mouse monoclonal anti-AOP1 antibody (A7674) at a 1:10000 dilution.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Mouse monoclonal anti-AOP1 antibody can be used for indirect ELISA and microarray applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

AOP1 (antioxidant-like protein 1) is also proposed to be involved in the synthesis of glucosinolate. It defends oxidative stress and participates in proliferation and differentiation through antioxidant function and redox regulation. Monoclonal antibodies reacting specifically with Aop1 are useful tools for the determination of the functional activity of Aop1.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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