A7921 Sigma-Aldrich


suitable for plant cell culture, powder

Synonym: Agar-agar, Gum agar



Related Categories Biochemicals and Reagents, Carbohydrates, Core Bioreagents, Culture Media Base Ingredient - Agar, Gelling Agents,
Quality Level   200
biological source   algae (Rhodophyceae)
form   powder
application(s)   cell culture | plant: suitable
transition temp   congealing temperature 30-35 °C
Featured Industry   Agriculture
storage temp.   room temp


General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.


Agar has been used as a component
• of Gamborg′s B5 regeneration medium
• of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
• in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings


1 kg in poly bottle

100, 500 g in poly bottle

2.5 kg in poly drum

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Gelling Agents - Plant Tissue Culture Protocol

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Keywords: Cell culture, Centrifugation, Immobilization, Inductively coupled plasma, Reductions

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