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A7921 Sigma-Aldrich

Agar

plant cell culture tested, powder

Synonym: Agar-agar, Gum agar

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Properties

Related Categories Carbohydrates, Core Bioreagents, Gelling Agents, Molecular Biology, Plant Biotechnology,
form   powder
transition temp   congealing temperature 30-35 °C
suitability   plant cell culture tested
Featured Industry   Agriculture

Description

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component
• of Gamborg′s B5 regeneration medium
• of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
• in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

2.5 kg in poly drum

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AW7950000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Protocols

Gelling Agents - Plant Tissue Culture Protocol

Agar has long been used to solidify media for plant tissue culture. The type of agar or gelling agent used can influence the growth of the tissue in culture. Both purity and cost of the gelling agent...
Keywords: Cell culture, Centrifugation, Immobilization, Inductively coupled plasma, Reductions

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Peer-Reviewed Papers
15

References

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