A8185 Sigma-Aldrich


BioReagent, suitable for cell culture, suitable for insect cell culture

Synonym: Alanyl-glutamine, Glutamine-S

  • CAS Number 39537-23-0

  • Empirical Formula (Hill Notation) C8H15N3O4

  • Molecular Weight 217.22

  •  MDL number MFCD00133046

  •  PubChem Substance ID 24891211

  •  NACRES NA.75



Related Categories Amino Acids, Amino Acids and Vitamins, Cell Culture, Companion Products and Reagents, Insect Platform,
Quality Level   200
product line   BioReagent
assay   ≥98% (HPLC)
form   powder
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
color   white to off-white
storage temp.   room temp
SMILES string   NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O
InChI   1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1



Ala-Gln has been used:
• as a supplement in NeuralQ medium
• as a component in growth medium and incubation medium (IM) for retinal cell lines
• for experimental treatment of retinal cell lines respectively


1 kg in poly drum

5, 100 g in poly bottle

Biochem/physiol Actions

Alanyl-glutamine (Ala-Gln) is a dipeptide that is used as a substitute for glutamine in mammalian cell culture media, because of the instability of glutamine in solution and the formation of ammonia as a breakdown product of glutamine in solution, which can have deleterious effects on cells in culture. Ala-Glu is stable to heat sterilization and is less ammonia genic than glutamine, which contributes to its advantages as a media component. Ala-Gln has been utilized to investigate polymorphonuclear leucocyte and myeloperoxidase activity in vitro.

Analysis Note

This product is cell culture tested (0.5 mg/ml) and insect cell culture tested (0.2 mg/ml). It is appropriate for use in cell culture and insect cell culture applications at 2-10 mM.

Legal Information

NeuralQ is a trademark of GlobalStem

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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