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  • A8228 - Anti-hABH2 antibody, Mouse monoclonal

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A8228 Sigma-Aldrich

Anti-hABH2 antibody, Mouse monoclonal

clone hABH2-7, purified from hybridoma cell culture

Synonym: Anti-Human AlkB Homologue 2, Monoclonal Anti-hABH2 antibody produced in mouse

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Properties

Related Categories A1-AB, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   hABH2-7, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using total cell extract of 293T cells
species reactivity   human
mol wt   antigen ~30 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   Q6NS38
Gene Information   human ... ALKBH2(121642)

Description

General description

The antibody does not recognize hABH1 or hABH3. Mouse Monoclonal Anti-hABH2 antibody binds to amino acids 8-21 of human hABH2.

Application

Mouse Monoclonal Anti-hABH2 antibody has been used for western blot assays. The antibody can also be used for IHC, ELISA, microarray, and immunoprecipitation studies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

hABH2 is a Fe (II) dependent dioxygenase involved in the repair of alkylation-induced damage in double stranded DNA. Alterations in hABH2 have been linked to pediatric brain tumor and gastric cancer.

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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