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  • A8273 - Anti-α-Amylase antibody produced in rabbit

A8273 Sigma-Aldrich

Anti-α-Amylase antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym: Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit, Alpha Amylase Antibody



Related Categories AM-AN, Alphabetical Index, Antibodies, Miscellaneous, Primary Antibodies More...
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   Ouchterlony double diffusion: suitable
  indirect ELISA: 1:4,000-1:6,000
species reactivity   human
form   lyophilized powder
storage temp.   2-8°C
packaging   vial of 2 mL lyophilized antiserum
antibody form   fractionated antiserum
Quality Level   200
antibody product type   primary antibodies
Gene Information   human ... AMY1A(276)


General description

α-amylase is primarily produced in abundance in the salivary glands and pancreas. The expression is also seen in jejunum and mammary glands. The genes encoding α-amylase are AMY1 and AMY2 located in the short arm of chromosome 1. AMY1 is responsible for producing the enzyme in saliva and mammary gland and AMY2 produces the enzyme synthesized in the pancreas. The expression of AMY1 occurs also in certain tumour tissues. Mammalian amylases are composed of three structural domains (A, B and C). Domain A contains the active site with two aspartate and one glutamate residue. Domain B borders the active site region and is essential for maintaining the protein conformation. Domain C is involved in catalytic mechanism.


The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. No reaction with other human saliva proteins or pancreatic extract proteins is observed.


human salivary α-amylase.


Anti-a-Amylase antibody produced in rabbit has been used in immunofluorescence and immunohistochemical analysis.

Anti-α-Amylase antibody produced in rabbit has also been used in immunocytochemistry analysis.

Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2


Reconstitute with 2 mL deionized water.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

α-Amylase is essential for catalyzing the primary step in starch digestion, a main source of carbohydrate in the human diet. α-amylase hydrolysis the polysaccharide with the ultimate production of maltose, maltotriose and limit dextrins as the main products.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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