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A8452 Sigma-Aldrich

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse

ascites fluid, clone C3

Synonym: Afp Antibody - Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, Afp Antibody, Alpha Fetoprotein Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Pluripotent Stem Cells,
conjugate   unconjugated
clone   C3, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human fetal liver
  indirect ELISA: suitable
species reactivity   canine, pig, human
should not react with   rat, mouse, cat, bovine
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P02771
Gene Information   human ... AFP(174)

Description

General description

Monoclonal Anti-α-Fetoprotein (AFP) (mouse IgG2a isotype) is derived from the C3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human α-fetoprotein.

α-Fetoprotein (AFP) is a one of the main glycoprotein present in the fetal liver, gastrointestinal tract, and yolk sac. AFP levels in amniotic fluid have been used as biomarkers for anencephaly and spina bifida.

Immunogen

human α-fetoprotein

Application

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse has been used in:
• enzyme-linked immunosorbent assay (ELISA)
• dot blot
• immunocytochemistry
• immunofluorescence
• immunocytochemistry

Mouse Monoclonal Anti-α-Fetoprotein has been used for immunocytochemistry. The product can also be used for dot blot and indirect ELISA.

Target description

AFP is a plasma protein found highly expressed in the human fetus where it functions to block the transport of estradiol across the placenta.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Increased concentrations of α-fetoprotein (AFP) in serum are less common in patients with malignancies of the gastrointestinal tract and of other organ systems with massive hepatic metastases. Hence, AFP determination is useful for the diagnosis of certain germ line tumors that contain yolk sac structures (testicular and ovarian tumors), hepatocellular carcinoma (hepatoma), and some malignant tumors of the gastrointestinal tract. AFP serves as an important marker for certain genetic and embryonic defects. Routine screening of pregnancies with a risk of neural tube defects (spina bifida, anencephaly, and Down′s syndrome) may be facilitated by the monitoring of AFP levels.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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