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A8792 Sigma-Aldrich

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
conjugate   peroxidase conjugate
clone   polyclonal
biological source   rabbit
application(s)   direct ELISA: 1:40,000
  dot blot: 1:80,000 (chemiluminescent)
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   secondary antibodies

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Specificity

Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogen

purified human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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