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A8806 Sigma-Aldrich

Bovine Serum Albumin

fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins, Albumins and Transport Proteins, Albumins by Application, Bioactives and Supplements for Stem Cell Expansion,
Quality Level   PREMIUM
biological source   bovine
product line   BioReagent
assay   ≥96% (agarose gel electrophoresis)
form   lyophilized powder
mol wt   ~66 kDa
purified by   cold ethanol fractionation
origin   USA origin
free fatty acid content   ≤0.02%
application(s)   cell culture | mammalian: suitable
impurities   ≤0.02% fatty acid (GC)
  ≤0.1 ng/mg endotoxin
loss   ≤5%
pH   4.8-7.5
solubility   water: soluble (40 mg/ml)
storage temp.   2-8°C

Description

General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Select the right albumin for your application with the Albumin Selection Guide

Bovine Serum Albumin has been used in the solution prepared for digestion of tissues. It has been used as a blocking agent in cell spreading assay.

Packaging

1, 5 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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